CDK4 Antibody (8F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26664

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDK4 Antibodies »

Product Summary for CDK4 Antibody (8F2)

Immunogen

A synthesized peptide derived from Human CDK4 [UniProt P11802]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDK4 Antibody (8F2)

CDK4 Antibody (8F2)

Immunoprecipitation: CDK4 Antibody (8F2) [NBP3-26664] -

Immunoprecipitation: CDK4 Antibody (8F2) [NBP3-26664] - Immunoprecipitating CDK4 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26664 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500).
Lane 2:NBP3-26664 (2ug)+ Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
CDK4 Antibody (8F2)

Western Blot: CDK4 Antibody (8F2) [NBP3-26664] -

Western Blot: CDK4 Antibody (8F2) [NBP3-26664] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate.
All lanes: CDK4 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 34, 21 kDa
Observed band size: 34 kDa

Applications for CDK4 Antibody (8F2)

Application
Recommended Usage

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CDK4

Cdk4 is encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Gene Symbol

CDK4

Additional CDK4 Products

Product Documents for CDK4 Antibody (8F2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CDK4 Antibody (8F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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