CDK4 Antibody (DCS-31)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-6315

Novus Biologicals, part of Bio-Techne
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NB120-6315
NB120-6315-0.05ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microarray, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # DCS-31

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CDK4 Antibodies »

Product Specifications

Immunogen

Recombinant human Cdk4 protein

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic (likely)

Specificity

Reacts specifically with Cdk4. Does not recognize other Cdk types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDK4 Antibody (DCS-31)

Western Blot: CDK4 Antibody (DCS-31) [NB120-6315]

Western Blot: CDK4 Antibody (DCS-31) [NB120-6315]

Western Blot: CDK4 Antibody (DCS-31) [NB120-6315] - WB analysis of lysates from human HeLa and SKUT cells using CDK4 antibody (clone DCS-31).

Applications for CDK4 Antibody (DCS-31)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
WB: recognizes a 33kD band, and it may pick up additional weaker bands, using a cultured human tumor cell line extract.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.9% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK4

Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Entrez Gene IDs

1019 (Human); 12567 (Mouse); 94201 (Rat)

Gene Symbol

CDK4

UniProt

P11802

Additional CDK4 Products

Product Documents for CDK4 Antibody (DCS-31)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDK4 Antibody (DCS-31)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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