CDK4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32288

Novus Biologicals, part of Bio-Techne
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NBP2-32288-100ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all CDK4 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within the following C-term region: PRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for CDK4 Antibody

Western Blot: CDK4 Antibody [NBP2-32288]

Western Blot: CDK4 Antibody [NBP2-32288]

Western Blot: CDK4 Antibody [NBP2-32288] - Reccomended Titration: 1.0 ug/ml Positive Control: HepG2 Whole Cell
Immunohistochemistry: CDK4 Antibody [NBP2-32288]

Immunohistochemistry: CDK4 Antibody [NBP2-32288]

Immunohistochemistry: CDK4 Antibody [NBP2-32288] - Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X
Western Blot: CDK4 Antibody [NBP2-32288]

Western Blot: CDK4 Antibody [NBP2-32288]

Western Blot: CDK4 Antibody [NBP2-32288] - Lanes: 1. 30 ug human HCT116 cell lysate 2. 30 ug genotoxic treated human HCT116 cell lysate 3. 30 ug genotoxic treated human HCT116 cell lysate Primary, Antibody Dilution: 1 : 2000 Secondary Antibody: Anti-rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene name: CDK4.
View 1 more images

Applications for CDK4 Antibody

Application
Recommended Usage

Immunohistochemistry

4-8 ug/ml

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK4

Cdk4 is encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Gene Symbol

CDK4

UniProt

P11802

Additional CDK4 Products

Product Documents for CDK4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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