Cdk6 Antibody (8G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26663

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26663-100ul
NBP3-26663-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cdk6 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Cdk6 [UniProt Q00534]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdk6 Antibody (8G3)

Cdk6 Antibody (8G3)

Immunoprecipitation: Cdk6 Antibody (8G3) [NBP3-26663] -

Immunoprecipitation: Cdk6 Antibody (8G3) [NBP3-26663] - Immunoprecipitating Cdk6 in K562 whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26663 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26663 (2ug)+ K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
Cdk6 Antibody (8G3)

Western Blot: Cdk6 Antibody (8G3) [NBP3-26663] -

Western Blot: Cdk6 Antibody (8G3) [NBP3-26663] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate.
All lanes: Cdk6 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 37 kDa
Observed band size: 37 kDa

Applications for Cdk6 Antibody (8G3)

Application
Recommended Usage

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Cdk6

Cdk6 is encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.

Long Name

Cyclin-dependent kinase 6

Alternate Names

CDKN6

Gene Symbol

CDK6

Additional Cdk6 Products

Product Documents for Cdk6 Antibody (8G3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cdk6 Antibody (8G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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