Cdk6 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92967
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 226-326 of human Cdk6 (NP_001250.1). DQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cdk6 Antibody - Azide and BSA Free
Western Blot: Cdk6 Antibody [NBP2-92967] -
Western Blot: Cdk6 Antibody [NBP2-92967] - analysis of lysates from HeLa cells, using CDK6 Rabbit pAb at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25Ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin-Cdk6 Antibody - Azide and BSA Free-NBP2-92967-
Immunohistochemistry-Paraffin-Cdk6 Antibody - Azide and BSA Free-NBP2-92967-y analysis of CDK6 inparaffin-embedded human coloncarcinoma using CDK6 Rabbit pAb at dilution of 1:100 (40x lens).Perform highpressure antigen retrieval with 10 mMcitrate buffer pH 6.0 before commencingwith IHC staining protocol.
Immunoprecipitation-Cdk6 Antibody - Azide and BSA Free-NBP2-92967-
Immunoprecipitation-Cdk6 Antibody - Azide and BSA Free-NBP2-92967-Analysis of 200 μg extracts of Jurkat cells, using 3 μg CDK6 antibody.Western blot was performed from the immunoprecipitate using CDK6 antibody at a dilutionof 1:1000.Applications for Cdk6 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Immunoprecipitation
0.5μg-4μg
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
Azide and BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cdk6
Long Name
Cyclin-dependent kinase 6
Alternate Names
CDKN6
Gene Symbol
CDK6
Additional Cdk6 Products
Product Documents for Cdk6 Antibody - Azide and BSA Free
Product Specific Notices for Cdk6 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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