CDX2 Antibody (SY09-02)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66769

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SY09-02

Concentration

1 mg/ml

View all CDX2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human CDX2 aa 20-60. (SwissProt: Q99626 Human; Unigene: 64495 Rat)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDX2 Antibody (SY09-02)

Western Blot: CDX2 Antibody (SY09-02) [NBP2-66769]

Western Blot: CDX2 Antibody (SY09-02) [NBP2-66769]

Western Blot: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of CDX2 on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Predicted band size: 34 kDa Observed band size: 40 kDa
Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (SY09-02) [NBP2-66769]

Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (SY09-02) [NBP2-66769]

Immunocytochemistry/Immunofluorescence: CDX2 Antibody (SY09-02) [NBP2-66769] - Staining CDX2 in LOVO cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769]

Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769]

Immunohistochemistry-Paraffin: CDX2 Antibody (SY09-02) [NBP2-66769] - Analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-CDX2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for CDX2 Antibody (SY09-02)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:500

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

Additional CDX2 Products

Product Documents for CDX2 Antibody (SY09-02)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDX2 Antibody (SY09-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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