CEACAM5/CD66e Antibody (CB30) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58734

Novus Biologicals, part of Bio-Techne
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NB110-58734
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Key Product Details

Species Reactivity

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CB30

Format

BSA Free

Concentration

1.0 mg/ml

View all CEACAM-5/CD66e Antibodies »

Product Specifications

Immunogen

Human carcinoembryonic antigen (CEA; CEACAM5)

Specificity

The antibody CB30 recognizes CD66e (CEA; 180-200 kDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CEACAM5/CD66e Antibody (CB30) - BSA Free

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]

Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CB30) - BSA Free [NB110-58734]

Immunohistochemistry-Paraffin: CEACAM-5/CD66e Antibody (CB30) [NB110-58734] - Human colorectal carcinoma

Applications for CEACAM5/CD66e Antibody (CB30) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50
Application Notes
For paraffin sections use standard ABC technique with pretreatment in 0.1% pepsin in 0.1M HCl for 30 minutes at RT. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CEACAM-5/CD66e

The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas potential target of tumor imaging and drug targeting.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Entrez Gene IDs

1048 (Human)

Gene Symbol

CEACAM5

Additional CEACAM-5/CD66e Products

Product Documents for CEACAM5/CD66e Antibody (CB30) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEACAM5/CD66e Antibody (CB30) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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