CEACAM5/CD66e Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85742

Novus Biologicals, part of Bio-Techne
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NBP1-85742
NBP1-85742-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CEACAM-5/CD66e Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEACAM5/CD66e Antibody

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody [NBP1-85742]

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody [NBP1-85742]

Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody [NBP1-85742] - Analysis in human colon and liver tissues using NBP1-85742 antibody. Corresponding CEACAM5 RNA-seq data are presented for the same tissues.
Western Blot: CEACAM5/CD66e Antibody [NBP1-85742]

Western Blot: CEACAM5/CD66e Antibody [NBP1-85742]

Western Blot: CEACAM5/CD66e Antibody [NBP1-85742] - Analysis in human colon tissue.
Immunocytochemistry/ Immunofluorescence: CEACAM5/CD66e Antibody [NBP1-85742]

Immunocytochemistry/ Immunofluorescence: CEACAM5/CD66e Antibody [NBP1-85742]

Immunocytochemistry/Immunofluorescence: CEACAM5/CD66e Antibody [NBP1-85742] - Staining of human cell line HaCaT shows localization to plasma membrane. Antibody staining is shown in green.
View 4 more images

Applications for CEACAM5/CD66e Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 -1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-5/CD66e

Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Alternate Names

CD66e, CEA, CEACAM5

Entrez Gene IDs

1048 (Human)

Gene Symbol

CEACAM5

Additional CEACAM-5/CD66e Products

Product Documents for CEACAM5/CD66e Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEACAM5/CD66e Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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