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CEACAM8/CD66b Antibody (24H12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26660

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26660-100ul
NBP3-26660-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 24H12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CEACAM8/CD66b Antibody (24H12)

Immunogen

A synthesized peptide derived from Human CEACAM8/CD66b [UniProt P31997]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEACAM8/CD66b Antibody (24H12)

CEACAM8/CD66b Antibody (24H12)

Flow Cytometry: CEACAM8/CD66b Antibody (24H12) [NBP3-26660] -

Flow Cytometry: CEACAM8/CD66b Antibody (24H12) [NBP3-26660] - Overlay Peak curve showing A549 cells stained with CEACAM8/CD66b Antibody (24H12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for CEACAM8/CD66b Antibody (24H12)

Application
Recommended Usage

Flow Cytometry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CEACAM-8/CD66b

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Gene Symbol

CEACAM8

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (24H12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CEACAM8/CD66b Antibody (24H12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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