CEBP alpha Antibody (4E10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37501

Novus Biologicals, part of Bio-Techne
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NBP2-37501-0.025ml
NBP2-37501
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E10

Format

BSA Free

Concentration

1 mg/ml

View all CEBP alpha Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CEBP alpha expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CEBP alpha Antibody (4E10) - BSA Free

Western Blot: CEBP alpha Antibody (4E10)BSA Free [NBP2-37501]

Western Blot: CEBP alpha Antibody (4E10)BSA Free [NBP2-37501]

Western Blot: CEBP alpha Antibody (4E10) [NBP2-37501] - Western blot analysis using CEBPA mouse mAb against THP-1 (1)cell lysate.
Flow Cytometry: CEBP alpha Antibody (4E10) - BSA Free [NBP2-37501]

Flow Cytometry: CEBP alpha Antibody (4E10) - BSA Free [NBP2-37501]

Flow Cytometry: CEBP alpha Antibody (4E10) [NBP2-37501] - An intracellular stain was performed on HepG2 cells with NBP2-37501APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Western Blot: CEBP alpha Antibody (4E10)BSA Free [NBP2-37501]

Western Blot: CEBP alpha Antibody (4E10)BSA Free [NBP2-37501]

Western Blot: CEBP alpha Antibody (4E10) [NBP2-37501] - Western blot analysis using CEBPA mAb against human CEBPA (AA: 139-204) recombinant protein. (Expected MW is 32.7 kDa)
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Applications for CEBP alpha Antibody (4E10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP alpha

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Gene Symbol

CEBPA

Additional CEBP alpha Products

Product Documents for CEBP alpha Antibody (4E10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CEBP alpha Antibody (4E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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