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CGI-16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90275

Novus Biologicals, part of Bio-Techne
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NBP1-90275
NBP1-90275-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VMVARDSENKGPAFVNPLIPESPEEEELFRQGELNKGRRIAFSSTSLLKMAPSAEERTTIHEMFLSTLDPKTISFRSRVLPSNAVWME

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CGI-16 Antibody

Western Blot: CGI-16 Antibody [NBP1-90275]

Western Blot: CGI-16 Antibody [NBP1-90275]

Western Blot: CGI-16 Antibody [NBP1-90275] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: CGI-16 Antibody [NBP1-90275]

Immunocytochemistry/ Immunofluorescence: CGI-16 Antibody [NBP1-90275]

Immunocytochemistry/Immunofluorescence: CGI-16 Antibody [NBP1-90275] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: CGI-16 Antibody [NBP1-90275]

Immunohistochemistry-Paraffin: CGI-16 Antibody [NBP1-90275]

Immunohistochemistry-Paraffin: CGI-16 Antibody [NBP1-90275] - Staining of human bronchus shows strong granular cytoplasmic positivity in respiratory epithelial cells.

Applications for CGI-16 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CGI-16

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fattyacid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs

Alternate Names

ACATE2, Acyl-CoA thioester hydrolase 9, acyl-CoA thioesterase 9acyl-Coenzyme A thioesterase 2, mitochondrial, acyl-coenzyme A thioesterase 9, mitochondrial, CGI-16, EC 3.1.2, EC 3.1.2.-, EC 3.1.2.15, mitochondrial Acyl-CoA Thioesterase, MT-ACT48

Gene Symbol

ACOT9

Additional CGI-16 Products

Product Documents for CGI-16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CGI-16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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