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CHAF1B Antibody (SS 24 1-68)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-211

Novus Biologicals, part of Bio-Techne
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NB500-211SS
NB500-211

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SS 24 1-68

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Recombinant human CHAF1B [UniProt# Q13112]

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHAF1B Antibody (SS 24 1-68)

Western Blot: CHAF1B Antibody (SS 24 1-68) [NB500-211]

Western Blot: CHAF1B Antibody (SS 24 1-68) [NB500-211]

Western Blot: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Analysis of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates using NB500-211.
Immunocytochemistry/ Immunofluorescence: CHAF1B Antibody (SS 24 1-68) [NB500-211]

Immunocytochemistry/ Immunofluorescence: CHAF1B Antibody (SS 24 1-68) [NB500-211]

Immunocytochemistry/Immunofluorescence: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Immunocytochemical staining of CAF-1 p60 in HeLa cells (Smith and Stillman 1991).

Applications for CHAF1B Antibody (SS 24 1-68)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

0.1-1 ug

Western Blot

1:1000
Application Notes
This CHAF1B (SS 24 1-68) antibody is useful for Immunoprecipitation, Western blot and Immunocytochemistry/Immunofluorescence. In WB a band can be seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CHAF1B

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Alternate Names

CAF1, CAF-1, CAF1ACAF-1 subunit B, CAF1P60CAF-I 60 kDa subunit, CAF-IP60, chromatin assembly factor 1 subunit B, chromatin assembly factor 1, subunit B (p60), Chromatin assembly factor I p60 subunit, human chromatin assembly factor-I p60 subunit, 10, MPHOSPH7CAF-I p60, MPP7M-phase phosphoprotein 7

Entrez Gene IDs

8208 (Human)

Gene Symbol

CHAF1B

UniProt

Additional CHAF1B Products

Product Documents for CHAF1B Antibody (SS 24 1-68)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHAF1B Antibody (SS 24 1-68)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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