CHCHD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83656

Novus Biologicals, part of Bio-Techne
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NBP1-83656
NBP1-83656-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CHCHD3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHCHD3 Antibody

Western Blot: CHCHD3 Antibody [NBP1-83656]

Western Blot: CHCHD3 Antibody [NBP1-83656]

Western Blot: CHCHD3 Antibody [NBP1-83656] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CHCHD3 Antibody [NBP1-83656]

Immunocytochemistry/ Immunofluorescence: CHCHD3 Antibody [NBP1-83656]

Immunocytochemistry/Immunofluorescence: CHCHD3 Antibody [NBP1-83656] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-83656]

Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-83656]

Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP1-83656] - Staining of human skeletal muscle shows strong granular cytoplasmic positivity in myocytes.
View 5 more images

Applications for CHCHD3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-83656 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHCHD3

Alternate Names

coiled-coil-helix-coiled-coil-helix domain containing 3, FLJ20420, mitochondrial

Gene Symbol

CHCHD3

Additional CHCHD3 Products

Product Documents for CHCHD3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CHCHD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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