CHCHD3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38566
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).
Sequence:
MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG
Sequence:
MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CHCHD3 Antibody
Immunoprecipitation: CHCHD3 Antibody [NBP3-38566] -
Immunoprecipitation: CHCHD3 Antibody [NBP3-38566] - Immunoprecipitation analysis of 600 ug extracts of Mouse liver cells using 3 ug CHCHD3 antibody. Western blot was performed from the immunoprecipitate using CHCHD3 antibody at a dilution of 1:1000.
Immunocytochemistry/ Immunofluorescence: CHCHD3 Antibody [NBP3-38566] -
Immunocytochemistry/ Immunofluorescence: CHCHD3 Antibody [NBP3-38566] - Confocal immunofluorescence analysis of U2OS cells using CHCHD3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: CHCHD3 Antibody [NBP3-38566] -
Western Blot: CHCHD3 Antibody [NBP3-38566] - Western blot analysis of various lysates using CHCHD3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.
Applications for CHCHD3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunoprecipitation
0.5ug-4ug antibody for 400ug-600ug extracts of whole cells
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CHCHD3
Alternate Names
coiled-coil-helix-coiled-coil-helix domain containing 3, FLJ20420, mitochondrial
Gene Symbol
CHCHD3
Additional CHCHD3 Products
Product Documents for CHCHD3 Antibody
Product Specific Notices for CHCHD3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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