CHD4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-57519
![Western Blot: CHD4 Antibody [NB100-57519]](https://resources.bio-techne.com/images/products/CHD4-Antibody-Western-Blot-NB100-57519-img0005.jpg "Western Blot: CHD4 Antibody [NB100-57519]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation Sequencing, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human chromodomain helicase DNA binding protein 4 (Mi2 autoantigen 218 kDa protein) using the numbering given in entry NP_001264.2 (GeneID 1108).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CHD4 Antibody
Western Blot: CHD4 Antibody [NB100-57519]
Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: CHD4 Antibody [NB100-57519]](https://resources.bio-techne.com/images/products/CHD4-Antibody-Western-Blot-NB100-57519-img0004.jpg "Western Blot: CHD4 Antibody [NB100-57519]")
Western Blot: CHD4 Antibody [NB100-57519]
Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: CHD4 Antibody [NB100-57519]](https://resources.bio-techne.com/images/products/CHD4-Antibody-Immunoprecipitation-NB100-57519-img0006.jpg "Immunoprecipitation: CHD4 Antibody [NB100-57519]")
Immunoprecipitation: CHD4 Antibody [NB100-57519]
Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.Applications for CHD4 Antibody
Application
Recommended Usage
Immunoprecipitation
2- 10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CHD4
Alternate Names
ATP-dependent helicase CHD4, CHD-4, chromodomain helicase DNA binding protein 4, chromodomain-helicase-DNA-binding protein 4, DKFZp686E06161, EC 3.6.1, EC 3.6.4.12, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-beta
Gene Symbol
CHD4
UniProt
Additional CHD4 Products
Product Documents for CHD4 Antibody
Product Specific Notices for CHD4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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