CHD4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57521

Novus Biologicals, part of Bio-Techne
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NB100-57521
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CHD4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1625 and 1675 of human chromodomain helicase DNA binding protein 4 (Mi2 autoantigen 218 kDa protein) using the numbering given in entry NP_001264.2 (GeneID 1108).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD4 Antibody

Western Blot: CHD4 Antibody [NB100-57521]

Western Blot: CHD4 Antibody [NB100-57521]

Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.
Immunoprecipitation: CHD4 Antibody [NB100-57521]

Immunoprecipitation: CHD4 Antibody [NB100-57521]

Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.

Applications for CHD4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in CHIP reported in scientific literature (PMID:31934863).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHD4

CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. Alternate names for CHD4/Mi2beta include chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b, and DKFZp686E06161.

Alternate Names

ATP-dependent helicase CHD4, CHD-4, chromodomain helicase DNA binding protein 4, chromodomain-helicase-DNA-binding protein 4, DKFZp686E06161, EC 3.6.1, EC 3.6.4.12, Mi-2 autoantigen 218 kDa protein, Mi-2b, Mi2-beta

Entrez Gene IDs

1108 (Human); 107932 (Mouse)

Gene Symbol

CHD4

UniProt

Q14839 (Human)

Additional CHD4 Products

Product Documents for CHD4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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