ChemR23/CMKLR1 Antibody (BZ194) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11984

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP3-11984AF350

Alexa Fluor 405

NBP3-11984AF405

Alexa Fluor 488

NBP3-11984AF488

Alexa Fluor 532

NBP3-11984AF532

Alexa Fluor 594

NBP3-11984AF594

Alexa Fluor 647

NBP3-11984AF647

Alexa Fluor 700

NBP3-11984AF700

Alexa Fluor 750

NBP3-11984AF750

Allophycocyanin

NBP3-11984APC

Biotin

NBP3-11984B

CoraFluor 1

NBP3-11984CL1

DyLight 350

NBP3-11984UV

DyLight 405

NBP3-11984V

DyLight 488

NBP3-11984G

DyLight 550

NBP3-11984R

DyLight 594

NBP3-11984DL594

DyLight 650

NBP3-11984C

DyLight 680

NBP3-11984FR

DyLight 755

NBP3-11984IR

FITC

NBP3-11984F

HRP

NBP3-11984H

Janelia Fluor 525

NBP3-11984JF525

Janelia Fluor 549

NBP3-11984JF549

Janelia Fluor 585

NBP3-11984JF585

Janelia Fluor 635

NBP3-11984JF635

Janelia Fluor 646

NBP3-11984JF646

Janelia Fluor 669

NBP3-11984JF669

mFluor Violet 450 SE

NBP3-11984MFV450

mFluor Violet 500 SE

NBP3-11984MFV500

mFluor Violet 610 SE

NBP3-11984MFV610

PE

NBP3-11984PE

PerCP

NBP3-11984PCP

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BZ194

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ChemR23 Antibodies »

Product Summary for ChemR23/CMKLR1 Antibody (BZ194) - Chimeric - Azide and BSA Free

Immunogen

This antibody was raised by immunising Wistar Furth rats with the mChemR23/CMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mChemR23/CMKLR1 peptide with the sequence NH2-DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8-32 of mChemR23/CMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Specificity

This antibody is specific for murine serpentine receptor mChemR23/CMKLR1 (aka ChemR23, DEZ). mChemR23/CMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin. Selective mChemR23/CMKLR1 expression has been demonstrated by mouse macrophages ex vivo, and by dendritic cell precursors cultured in vitro.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for ChemR23/CMKLR1 Antibody (BZ194) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ChemR23/CMKLR1 Antibody (BZ194) - Chimeric - Azide and BSA Free [NBP3-11984]

Immunocytochemistry/Immunofluorescence: ChemR23/CMKLR1 Antibody (BZ194) - Chimeric [NBP3-11984] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BZ194 (NBP3-11984) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11984, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for ChemR23/CMKLR1 Antibody (BZ194) - Chimeric - Azide and BSA Free

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: ChemR23

Chemokine-Like Receptor 1 (CMKLR1) is an chemoattractant receptor. CMKLR1 is thought to act primarily in bone development where it is differentially regulated in osseous and cartilaginous tissue. It also acts as a coreceptor for immunodeficiency viruses. CMKLR1 has two variants that are produced by alternative splicing. Chemokine-like receptor 1 (CMKLR1) has been reported to be expressed in bone marrow, liver, appendix, thymus, lymph node, placenta, and spleen. ESTs have been isolated from human normal kidney and testis libraries and from a prostate cancer library.

Long Name

Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23

Alternate Names

cmklr1, Dez, Gpcr27

Gene Symbol

CMKLR1

Additional ChemR23 Products

Product Documents for ChemR23/CMKLR1 Antibody (BZ194) - Chimeric - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for ChemR23/CMKLR1 Antibody (BZ194) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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