CHIP/STUB1 Antibody (034)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90116

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90116-100ul
NBP2-90116-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 034

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CHIP/STUB1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CHIP/STUB1 isoform 1 (Uniprot#: Q9UNE7-1; Met 1-Tyr 303).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CHIP/STUB1 Antibody (034)

Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116]

Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116]

Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.
Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116]

Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116]

Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116] - Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human STUB1 monoclonal antibody (dilution ratio 1:300) overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.
Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116]

Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116]

Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116] - Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Applications for CHIP/STUB1 Antibody (034)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:100-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHIP/STUB1

STUB1/CHIP is an E3 ubiquitin ligase and co-chaperone of heat-shock protein 70 (Hsp70). STUB1/CHIP interacts with Hsp70 to monitor protein folding and direct misfolded proteins for proteasomal degradation. It has been found to also associate with Hsp90 to remove phosphorylated protein tau, a protein that accumulates in the brains of Alzheimers and Down's syndrome patients. STUB1/CHIP has been reported to also have intrinsic chaperone activity and recognize non-native proteins in response to heat stress. In this way, STUB1/CHIP has been shown to be a direct chaperone for p53. Alternate names for STUB1/CHIP include carboxy terminus of Hsp70-interacting protein, STIP1 homology and U-box containing protein 1, CLL-associated antigen KW-8 antigen, serologically defined colon cancer antigen 7, heat shock protein A binding protein 2, NY-CO-7, HSPABP2, and SDCCAG7.

Long Name

C-terminus of Hsc70 Interacting Protein

Alternate Names

HSPABP2, NY-CO-7, SDCCAG7, STUB1, UBOX1

Gene Symbol

STUB1

Additional CHIP/STUB1 Products

Product Documents for CHIP/STUB1 Antibody (034)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CHIP/STUB1 Antibody (034)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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