Chk1 Antibody (2G1D5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1768

Novus Biologicals, part of Bio-Techne
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NB100-1768
NB100-1768SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2G1D5

Format

BSA Free

Concentration

1.0 mg/ml

View all Chk1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Chk1 expressed in E. coli. [UniProt# O14757]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chk1 Antibody (2G1D5) - BSA Free

Western Blot: Chk1 Antibody (2G1D5)BSA Free [NB100-1768]

Western Blot: Chk1 Antibody (2G1D5)BSA Free [NB100-1768]

Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).
Immunocytochemistry/ Immunofluorescence: Chk1 Antibody (2G1D5) - BSA Free [NB100-1768]

Immunocytochemistry/ Immunofluorescence: Chk1 Antibody (2G1D5) - BSA Free [NB100-1768]

Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.
Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) - BSA Free [NB100-1768]

Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) - BSA Free [NB100-1768]

Immunohistochemistry-Paraffin: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis of FFPE human lung using Chk1 antibody (clone 2G1D5) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and nuclear staining of Chk1 was observed as this is a ubiquitous cell checkpoint protein. Staining was performed by Histowiz.
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Applications for Chk1 Antibody (2G1D5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Simple Western

1:100

Western Blot

1:200 - 1:1000
Application Notes
See Simple Western Antibody Database for Simple Western validation: tested in transfected HeLa, human primary fibroblast lysates; antibody dilution of 1:100; separated by size

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chk1

Chk1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by Chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Entrez Gene IDs

1111 (Human)

Gene Symbol

CHEK1

UniProt

O14757

Additional Chk1 Products

Product Documents for Chk1 Antibody (2G1D5) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Chk1 Antibody (2G1D5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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