Chk1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-311
![Western Blot: Chk1 Antibody [NB100-311]](https://resources.bio-techne.com/images/products/Chk1-Antibody-Western-Blot-NB100-311-img0200.jpg "Western Blot: Chk1 Antibody [NB100-311]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The epitope recognized by NB100-311 maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Chk1 Antibody - BSA Free
Western Blot: Chk1 Antibody [NB100-311]
Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: Chk1 Antibody [NB100-311]](https://resources.bio-techne.com/images/products/Chk1-Antibody-Western-Blot-NB100-311-img0003.jpg "Western Blot: Chk1 Antibody [NB100-311]")
Western Blot: Chk1 Antibody [NB100-311]
Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.Applications for Chk1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
5-20 ug/mg lysate
Western Blot
1:1000-1:10000
Application Notes
100-311 may be used for Western Blot where a band at ~53 kD is seen on SDS-PAGE. It may also be used for immunoprecipitation (band ~ 48 kDa). Suggested working dilutions: * Western Blot 1:1,000-1:10,000 (ECL) Immunocytochemistry Not Done Immunoprecipitation 5-20 ug/g lysate *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Chk1
Additional Chk1 Products
Product Documents for Chk1 Antibody - BSA Free
Product Specific Notices for Chk1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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