Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all Chk1 Antibodies »

Product Specifications

Immunogen

The epitope recognized by NB100-311 maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Chk1 Antibody

Western Blot: Chk1 Antibody [NB100-311]

Western Blot: Chk1 Antibody [NB100-311]

Western Blot: Chk1 Antibody [NB100-311] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Chk1 Antibody [NB100-311]

Western Blot: Chk1 Antibody [NB100-311]

Western Blot: Chk1 Antibody [NB100-311] - Whole Cell lysate (50 ug for WB, 500 ug for IP) from HeLa cells. Affinity purified goat anti-Chk1 used as indicated. Immunoprecipitated material was detected by WB using a monoclonal antibody to Chk1. Detection: Chemiluminescence with 1 minute exposure.

Applications for Chk1 Antibody

Application
Recommended Usage

Immunoprecipitation

5-20 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
100-311 may be used for Western Blot where a band at ~53 kD is seen on SDS-PAGE. It may also be used for immunoprecipitation (band ~ 48 kDa). Suggested working dilutions: * Western Blot 1:1,000-1:10,000 (ECL) Immunocytochemistry Not Done Immunoprecipitation 5-20 ug/g lysate *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Chk1

In response to DNA damage and replication blocks, cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 is an essential kinase for embryonic development and regulating mitotic progression in response to DNA damage.

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Entrez Gene IDs

1111 (Human)

Gene Symbol

CHEK1

Additional Chk1 Products

Product Documents for Chk1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Chk1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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