Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Chk1 Antibodies »

Product Summary for Chk1 [p Ser317] Antibody

Immunogen

This antibody maps to a region between residue Immunogen for A304-673A was a phosphorylated synthetic peptide, which represented a portion of human Checkpoint Kinase 1 (GeneID 1111) around serine 317 according to the numbering given in entry NP_001265.1. of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).

Modification

p Ser317

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Chk1 [p Ser317] Antibody

Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703]

Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703]

Western Blot: Chk1 [p Ser317] Antibody [NBP2-37703] - Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer treated with hydroxyurea (2mM for 24 hours), EPE (100uM for 4 hours), or untreated. Affinity purified rabbit anti-Chk1, Phospho (S317) antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: Chk1 [p Ser317] Antibody [NBP2-37703]

Immunohistochemistry: Chk1 [p Ser317] Antibody [NBP2-37703]

Immunohistochemistry: Chk1 [p Ser317] Antibody [NBP2-37703] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Chk1, Phospho (S317) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Applications for Chk1 [p Ser317] Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Chk1

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Gene Symbol

CHEK1

UniProt

NP_001265.1 (Human)

Additional Chk1 Products

Product Documents for Chk1 [p Ser317] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Chk1 [p Ser317] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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