CHMP4A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-79920

Novus Biologicals, part of Bio-Techne
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NBP1-79920
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all CHMP4A Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is CHMP4A. Peptide sequence ELEQEELAQELLNVGDKEEEPSVKLPSVPSTHLPAGPAPKVDEDEEALKQ. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for CHMP4A Antibody

Western Blot: CHMP4A Antibody [NBP1-79920]

Western Blot: CHMP4A Antibody [NBP1-79920]

Western Blot: CHMP4A Antibody [NBP1-79920] - 721_B Cell Lysate 1ug/ml Gel Concentration 12%

Applications for CHMP4A Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHMP4A

CHMP4A (charged MVB protein 4A) is one of the three isoforms of CHMP4, the human equivalent of yeast Snf7 (so CHMP4A is also referred to as hSnf7-1). It is a component of the ESCRT-III (endosomal sorting complex required for transport III) complex. The ESCRT complexes mediate membrane budding away from the cytosol and cleavage of membrane necks (membrane fission) from the cytosolic face; hence, they are key in processes such as the formation of MVBs (multivesicular bodies), enveloped virus budding, and membrane abscission in cytokinesis. Not all types of ESCRT complexes, or proteins within those complexes, are necessary for all of these functions. For instance, research into the requirements of ESCRT-III proteins in HIV-1 budding has revealed that CHMP1 and CHMP3 proteins are not essential for this process; at least one member of each of the CHMP2 and CHMP4 families are required; within these families there is some functional redundancy; within the CHMP4 family, the relative importance and abundance of the isoforms is 4B > 4A > 4C; and the absence of CHMP4A or 4B prevents the budding process from progressing to completion.

Alternate Names

C14orf123, charged multivesicular body protein 4a, CHMP4, CHMP4a, CHMP4B, chromatin modifying protein 4A, Chromatin-modifying protein 4a, chromosome 14 open reading frame 123, FLJ61658, hSnf-1, HSPC134, hVps32-1, MGC142093, MGC142095, SHAX2, SNF7, SNF7 homolog associated with Alix-2, Snf7 homologue associated with Alix 2, SNF7-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, VPS32A

Gene Symbol

CHMP4A

UniProt

NP_054888 (Human)

Additional CHMP4A Products

Product Documents for CHMP4A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CHMP4A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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