Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5C7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CHMP5 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CHMP5 (NP_057494) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHMP5 Antibody (OTI5C7) - Azide and BSA Free

Western Blot: CHMP5 Antibody (OTI5C7)Azide and BSA Free [NBP2-71937]

Western Blot: CHMP5 Antibody (OTI5C7)Azide and BSA Free [NBP2-71937]

Western Blot: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHMP5.
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937]

Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937]

Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937]

Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937]

Immunohistochemistry: CHMP5 Antibody (OTI5C7) - Azide and BSA Free [NBP2-71937] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
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Applications for CHMP5 Antibody (OTI5C7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHMP5

CHMP5 is a component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargoes of viral proteins.

Alternate Names

apoptosis-related protein PNAS-2, C9orf83, charged multivesicular body protein 5, chromatin modifying protein 5, Chromatin-modifying protein 5, chromosome 9 open reading frame 83, HSPC177, hVps60, PNAS-2, SNF7 domain containing 2, SNF7 domain-containing protein 2, SNF7DC2, Vacuolar protein sorting-associated protein 60, Vps60CGI-34

Gene Symbol

CHMP5

Additional CHMP5 Products

Product Documents for CHMP5 Antibody (OTI5C7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CHMP5 Antibody (OTI5C7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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