Choline Acetyltransferase/ChAT Antibody (1I4V0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15621
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1I4V0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Choline Acetyltransferase/ChAT Antibody (1I4V0)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 649-748 of human Choline Acetyltransferase/ChAT (P28329). MSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Choline Acetyltransferase/ChAT Antibody (1I4V0)
![Western Blot: Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621]](https://resources.bio-techne.com/images/products/Choline-Acetyltransferase-ChAT-Antibody-1I4V0-Western-Blot-NBP3-15621-img0002.jpg "Western Blot: Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621]")
Western Blot: Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621]
Western Blot: Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Western blot analysis of extracts of SH-SY5Y cells, using Choline Acetyltransferase/ChAT antibody (NBP3-15621) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Parafin-Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] -
Immunohistochemistry-Parafin-Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Mouse brain using CHAT Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
Immunohistochemistry-Paraffin-Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] -
Immunohistochemistry-Paraffin-Choline Acetyltransferase/ChAT Antibody (1I4V0) [NBP3-15621] - Rat brain using CHAT Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Applications for Choline Acetyltransferase/ChAT Antibody (1I4V0)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Choline Acetyltransferase/ChAT
Alternate Names
CHAT, Choline acetylase
Gene Symbol
CHAT
Additional Choline Acetyltransferase/ChAT Products
Product Documents for Choline Acetyltransferase/ChAT Antibody (1I4V0)
Product Specific Notices for Choline Acetyltransferase/ChAT Antibody (1I4V0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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