Choline Acetyltransferase/ChAT Antibody (8E7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26659
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8E7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Choline Acetyltransferase/ChAT [UniProt P28329]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Choline Acetyltransferase/ChAT Antibody (8E7)
Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] -
Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Image of Choline Acetyltransferase/ChAT Antibody (8E7) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.22% DAB.
Western Blot: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] -
Western Blot: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Positive Western Blot detected in: Mouse Brain tissue lysate.All lanes: Choline Acetyltransferase/ChAT Antibody at 1: 500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] -
Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (8E7) [NBP3-26659] - Staining of SH-SY5Y with Choline Acetyltransferase/ChAT Antibody (8E7) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 498-conjugated Goat Anti-Rabbit IgG (H+L).Applications for Choline Acetyltransferase/ChAT Antibody (8E7)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Choline Acetyltransferase/ChAT
Alternate Names
CHAT, Choline acetylase
Gene Symbol
CHAT
Additional Choline Acetyltransferase/ChAT Products
Product Documents for Choline Acetyltransferase/ChAT Antibody (8E7)
Product Specific Notices for Choline Acetyltransferase/ChAT Antibody (8E7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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