Choline Acetyltransferase/ChAT Antibody

Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052]

Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052]

Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Immunostaining of anterior horn of lumbar spinal cord for Choline Acetyltransferase/ChAT (in green) and p62 (in red) at 130 days. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (https://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201808888), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] -

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha-motor neurons & interneurons in the thoracic spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks thoracic spinal cord staining for alpha-motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma-motor neurons (Chat+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha-motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma-motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha-motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma-motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the thoracic spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] -

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - MATR3 loss in alpha-motor neurons & interneurons in the cervical spinal cord of Matr3S85C/S85C mice. (A) Representative images of 10 & 30 weeks cervical spinal cord staining for alpha-motor neurons (ChAT+, NeuN+, denoted by white arrows) & gamma-motor neurons (ChAT+, NeuN−, denoted by yellow arrows). Scale bar for the spinal cord image denotes 500 μm & the zoomed-in image denotes 50 µm. Quantification of the percentage of motor neurons with reduced MATR3 staining in (B) alpha-motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (C) gamma-motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Quantification of the number of (D) alpha-motor neurons (10 weeks: n = 6 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C) & (E) gamma-motor neurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (F) Representative images of 10 & 30 weeks PVALB+ interneurons in the cervical spinal cord. Interneurons with reduced MATR3 staining are denoted by a white asterisk. Scale bar denotes 50 µm. (G) Quantification of the percentage of PVALB+ interneurons with reduced MATR3 staining (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). (H) Quantification of the number of PVALB+ interneurons (10 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C; 30 weeks: n = 3 Matr3+/+, 3 Matr3S85C/S85C). Bar graph heights depict mean ± SEM, with each datapoint representing an animal. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35205163), licensed under a CC-BY license. Not internally tested by Novus Biologicals.