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Choline Kinase beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28466

Novus Biologicals, part of Bio-Techne
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NBP1-28466

Key Product Details

Species Reactivity

Human, Canine

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KGETLSQEEQRKLEED corresponding to internal region according to NP_005189.2.

Epitope

C terminus

Specificity

This antibody is expected to recognize isoform a (NP_005189.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Applications for Choline Kinase beta Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 38 kDa band in human skeletal muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3 kDa band according to NP_005189.2. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Choline Kinase beta

Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus.

Alternate Names

CHETK, CHKL, ChoKB, CK, CKEKB, EK, EKB, Ethanolamine Kinase beta, MDCMC

Entrez Gene IDs

1120 (Human)

Gene Symbol

CHKB

Additional Choline Kinase beta Products

Product Documents for Choline Kinase beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Choline Kinase beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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