Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CPTC-CHP2-1

Concentration

0.2 mg/ml

View all Chp2 Antibodies »

Product Specifications

Immunogen

Recombinant human full-length Chp2 protein (Uniprot: O43745)

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08694)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Chp2 Antibody (CPTC-CHP2-1)

Western Blot: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819]

Western Blot: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819]

Western Blot: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819] - Western Blot Analysis of MCF-7 & PC3 cell lysates using Chp2 Mouse Monoclonal Antibody (CPTC-CHP2-1).
Immunocytochemistry/ Immunofluorescence: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819]

Immunocytochemistry/ Immunofluorescence: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819]

Immunocytochemistry/Immunofluorescence: Chp2 Antibody (CPTC-CHP2-1) [NBP3-07819] - Immunofluorescence Analysis of human MCF-7 cells labeling Chp2 with Chp2 Mouse Monoclonal Antibody (CPTC-Chp2-1) followed by Goat anti- Mouse IgG-CF488 (Green).The nuclear counterstain is RedDot (Red)

Applications for Chp2 Antibody (CPTC-CHP2-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Chp2

Chp2 binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cellsfrom serum deprivation-induced death

Alternate Names

calcineurin B homologous protein 2, HCA520, hepatocellular carcinoma antigen gene 520, Hepatocellular carcinoma-associated antigen 520

Gene Symbol

CHP2

UniProt

O43745 (Human)

Additional Chp2 Products

Product Documents for Chp2 Antibody (CPTC-CHP2-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Chp2 Antibody (CPTC-CHP2-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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