Chromogranin A Antibody (D11-2-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32185
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # D11-2-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human Chromogranin-A aa 415-457/457. (Uniprot: P10645)
Localization
Secreted. Secretory vesicle lumen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Chromogranin A Antibody (D11-2-R)
Immunohistochemistry: Chromogranin A Antibody (D11-2-R) [NBP3-32185]
Immunohistochemistry: Chromogranin A Antibody (D11-2-R) [NBP3-32185] - Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue with Mouse anti-Chromogranin A antibody (NBP3-32185) at 1/15,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32185) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for Chromogranin A Antibody (D11-2-R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:15000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chromogranin A
Alternate Names
Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I
Gene Symbol
CHGA
Additional Chromogranin A Products
Product Documents for Chromogranin A Antibody (D11-2-R)
Product Specific Notices for Chromogranin A Antibody (D11-2-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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