Chromogranin A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - SH-SY5Y cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Chromogranin A at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Mouse pancreas cryosections stained with insulin (DAKO, Green) and Chromogranin A (NB120-15160; 1:500; White)

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160]

Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] -

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells do not replicate during fetal & infant life. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), Ki67 (red), & DAPI (blue). Yellow arrows indicate CPHN cells. CPHN cells were rarely positive for Ki67 staining in both fetal & infant groups; no detectable difference was found in the frequency of replicative CPHN cells between fetal & infant pancreatic sections. Scale bars: 100 μm for low power & 50 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] -

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the beta-cell differentiation transcription factor NKX2.2 in both fetuses & infants. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX2.2 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] -

Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody - BSA Free [NB120-15160] - ChromograninA positive hormone-negative (CPHN) cells express the endocrine differentiation transcription factor NKX6.1 in both fetal & infant pancreas. Representative pancreatic sections from fetal (A) & infant (B) donors immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, & ghrelin) (white), chromograninA (green), the transcription factor NKX6.1 (red) & DAPI (blue). Yellow arrows indicate CPHN cells. Scale bars: 100 μm for low power & 25 μm for high magnification images. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30687234), licensed under a CC-BY license. Not internally tested by Novus Biologicals.