Skip to main content

CHSS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88087

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-88087
NBP1-88087-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GCTGDHEGVHYSHLELSPGEPVQEGDPHFRSALTAHPVRDPVHMYQLHKAFARAELERTYQEIQELQWEIQNTSHLAVDGDRAAAWPVGIPAPS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHSS2 Antibody

Western Blot: CHSS2 Antibody [NBP1-88087]

Western Blot: CHSS2 Antibody [NBP1-88087]

Western Blot: CHSS2 Antibody [NBP1-88087] - Analysis in control (vector only transfected HEK293T lysate) and CHPF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CHSS2 Antibody [NBP1-88087]

Immunocytochemistry/ Immunofluorescence: CHSS2 Antibody [NBP1-88087]

Immunocytochemistry/Immunofluorescence: CHSS2 Antibody [NBP1-88087] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, centrosome & vesicles.
Immunohistochemistry-Paraffin: CHSS2 Antibody [NBP1-88087]

Immunohistochemistry-Paraffin: CHSS2 Antibody [NBP1-88087]

Immunohistochemistry-Paraffin: CHSS2 Antibody [NBP1-88087] - Staining of human small intestine shows moderate cytoplasmic and membranous positivity in glandular cells.

Applications for CHSS2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHSS2

CHSS2 has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfersglucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end ofthe elongating chondroitin polymer

Alternate Names

Chondroitin glucuronyltransferase 2, chondroitin polymerizing factor, chondroitin sulfate synthase 2, Chondroitin-polymerizing factor, ChPF, CHSY2, CSS2FLJ22678, EC 2.4.1.175, EC 2.4.1.226, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II, N-acetylgalactosaminyltransferase 2

Gene Symbol

CHPF

Additional CHSS2 Products

Product Documents for CHSS2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHSS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...