CHTF18 Antibody (1F5)
Novus Biologicals, part of Bio-Techne | Catalog # H00063922-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CHTF18 (AAH18184, 886 a.a. ~ 975 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVHRPAPRNHEQRLEHIMRRAAREEQPEKDFFGRVVVRSTAVPSAGDTAPEQDSVERRMGTAVGRSEVWFRFNEGVSNAVRRSLYIRDLL
Reactivity Notes
Human. Other species not tested.
Specificity
CHTF18 - CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for CHTF18 Antibody (1F5)
![Western Blot: CHTF18 Antibody (1F5) [H00063922-M01]](https://resources.bio-techne.com/images/products/CHTF18-Antibody-1F5-Western-Blot-H00063922-M01-img0005.jpg "Western Blot: CHTF18 Antibody (1F5) [H00063922-M01]")
Western Blot: CHTF18 Antibody (1F5) [H00063922-M01]
Western Blot: CHTF18 Antibody (1F5) [H00063922-M01] - CHTF18 monoclonal antibody (M01), clone 1F5 Analysis of CHTF18 expression in HeLa.![Western Blot: CHTF18 Antibody (1F5) [H00063922-M01]](https://resources.bio-techne.com/images/products/CHTF18-Antibody-1F5-Western-Blot-H00063922-M01-img0006.jpg "Western Blot: CHTF18 Antibody (1F5) [H00063922-M01]")
Western Blot: CHTF18 Antibody (1F5) [H00063922-M01]
Western Blot: CHTF18 Antibody (1F5) [H00063922-M01] - Analysis of CHTF18 expression in transfected 293T cell line by CHTF18 monoclonal antibody (M01), clone 1F5.Lane 1: CHTF18 transfected lysate(107 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CHTF18 Antibody (1F5) [H00063922-M01] - Detection limit for recombinant GST tagged CHTF18 is approximately 0.1ng/ml as a capture antibody.
Applications for CHTF18 Antibody (1F5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 22493068)
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CHTF18
Alternate Names
C16orf41, C321D2.2, C321D2.3, C321D2.3 (novel protein), C321D2.4 (novel protein), CHL12C321D2.4, chromosome 16 open reading frame 41, chromosome transmission fidelity protein 18 homolog, CTF18, CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae), EC 3.6.1, hCTF18, homolog of yeast CHL12, RUVBL, some homology with holliday junction DNA helicase RUVB like
Entrez Gene IDs
63922 (Human)
Gene Symbol
CHTF18
UniProt
Additional CHTF18 Products
Product Documents for CHTF18 Antibody (1F5)
Product Specific Notices for CHTF18 Antibody (1F5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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