CIDEA Antibody (4B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00001149-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CIDEA Antibodies »

Product Specifications

Immunogen

CIDEA (AAH31896, 1 a.a. ~ 253 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRGDRASGGPGNHNGSWAREGPRLGPSWKRGLWSPRGGPNRPAEPSRPLTFMGSQTKRVLFTPLMHPARPFRVSNHDRSSRRGVMASSLQELISKTLDALVIATGLVTLVLEEDGTVVDTEEFFQTLGDNTHFMILEKGQKWMPGSQHVPTCSPPKRSGIARVTFDLYRLNPKDFIGCLNVKATMYEMYSVSYDIRCTGLKGLLRSLLRFLSYSAQVTGQFLIYLGTYMLRVLDDKEERPSLRSQAKGRFTCG

Specificity

CIDEA - cell death-inducing DFFA-like effector a

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CIDEA Antibody (4B9)

Western Blot: CIDEA Antibody (4B9) [H00001149-M01]

Western Blot: CIDEA Antibody (4B9) [H00001149-M01]

Western Blot: CIDEA Antibody (4B9) [H00001149-M01] - Analysis of CIDEA expression in transfected 293T cell line by CIDEA monoclonal antibody (M01), clone 4B9.Lane 1: CIDEA transfected lysate(28.3 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: CIDEA Antibody (4B9) [H00001149-M01]

Immunoprecipitation: CIDEA Antibody (4B9) [H00001149-M01]

Immunoprecipitation: CIDEA Antibody (4B9) [H00001149-M01] - Analysis of CIDEA transfected lysate using anti-CIDEA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CIDEA MaxPab rabbit polyclonal antibody.
ELISA: CIDEA Antibody (4B9) [H00001149-M01]

ELISA: CIDEA Antibody (4B9) [H00001149-M01]

ELISA: CIDEA Antibody (4B9) [H00001149-M01] - Detection limit for recombinant GST tagged CIDEA is 1 ng/ml as a capture antibody.

Applications for CIDEA Antibody (4B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. Use in Immunohistochemistry reported in scientific literature (PMID: 30089732).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CIDEA

This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Two alternative transcripts encoding different isoforms have been identified.

Long Name

Cell Death-inducing DFFA-like Effector A

Alternate Names

cell death activator CIDE-A, cell death-inducing DFFA-like effector aCIDE-A

Entrez Gene IDs

1149 (Human)

Gene Symbol

CIDEA

OMIM

604440 (Human)

UniProt

AAH31896 (Human)

Additional CIDEA Products

Product Documents for CIDEA Antibody (4B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CIDEA Antibody (4B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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