CIP2A Antibody (2G10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80670
![Flow Cytometry: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.bio-techne.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80670-img0005.jpg "Flow Cytometry: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
![Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]](https://resources.bio-techne.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Western-Blot-NBP2-80670-img0002.jpg "Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Primate
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2G10
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for CIP2A Antibody (2G10) - Azide and BSA Free
Immunogen
A synthetic peptide made to a C-terminal portion of the human CIP2A protein. [UniProt# Q8TCG1]
Epitope
A 300 amino acid portion of the C-terminus.
Reactivity Notes
Other species have not been tested.
Localization
Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for CIP2A Antibody (2G10) - Azide and BSA Free
Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]
Western Blot: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - Detection of CIP2A in HeLa whole cell lysate. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.bio-techne.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80670-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
Immunocytochemistry/ Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]
Immunocytochemistry/Immunofluorescence: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - CIP2A antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.bio-techne.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80670-img0004.jpg "Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]
Immunohistochemistry-Paraffin: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using CIP2A antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (![Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]](https://resources.bio-techne.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Western-Blot-NBP2-80670-img0001.jpg "Western Blot: CIP2A Antibody (2G10)Azide and BSA Free [NBP2-80670]")
![Knockdown Validated: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]](https://resources.bio-techne.com/images/products/CIP2A-Antibody-2G10-Azide-and-BSA-Free-Knockdown-Validated-NBP2-80670-img0006.jpg "Knockdown Validated: CIP2A Antibody (2G10) - Azide and BSA Free [NBP2-80670]")
Applications for CIP2A Antibody (2G10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:40
Immunohistochemistry
1:100 - 1:200
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 25965834)
Western Blot
1:1000-1:2000
Application Notes
In ICC/IF, strong cytoplasmic staining is observed in U20s cells. In Western Blot analysis, a band is seen at ~90 kDa representing CIP2A.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CIP2A
Alternate Names
Cancerous inhibitor of PP2A, CIP2A, FLJ12850, KIAA1524, MGC163436, p90, p90 autoantigen, protein CIP2A
Gene Symbol
CIP2A
Additional CIP2A Products
Product Documents for CIP2A Antibody (2G10) - Azide and BSA Free
Product Specific Notices for CIP2A Antibody (2G10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...