CITED4 Antibody (HT13-2D6.3)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-41572

Novus Biologicals, part of Bio-Techne
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NB110-41572
NB110-41572SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HT13-2D6.3

Concentration

1 mg/ml

View all CITED4 Antibodies »

Product Specifications

Immunogen

N-terminal fusion protein to full-length human CITED4 [UniProt# Q96RK1]

Localization

Cytoplasmic and Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CITED4 Antibody (HT13-2D6.3)

Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572]

Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572]

Western Blot: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Detection of human CITED4 in 20ug of transfected whole cell Hep3B lysate using NB110-415752. Photo courtesy of Dr. DeCaprio, DFCI.
Immunohistochemistry-Paraffin: CITED4 Antibody (HT13-2D6.3) [NB110-41572]

Immunohistochemistry-Paraffin: CITED4 Antibody (HT13-2D6.3) [NB110-41572]

Immunohistochemistry-Paraffin: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - CITED4 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CITED4 (HT13-2D6.3) Monoclonal Antibody (Catalog # NB110-41572) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in cardiomyocytes.
Flow Cytometry: CITED4 Antibody (HT13-2D6.3) [NB110-41572]

Flow Cytometry: CITED4 Antibody (HT13-2D6.3) [NB110-41572]

Flow Cytometry: CITED4 Antibody (HT13-2D6.3) [NB110-41572] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using CITED4 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Applications for CITED4 Antibody (HT13-2D6.3)

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

reported in scientific literature

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This CITED4 antibody is useful for CITED4 transfected cells. In Western blot a band was observed ~25 kDa. A lower (15 kDa) non-specific band may also be seen. Dilutions should be determined by the researcher.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CITED4

CITED4 (CREB-binding protein/p300-interacting transactivator with E/D-rich tail 4) belongs to CITED family and all members of this family share CITED domain that can interact with CBP and p300. These proteins are transcriptional co-activators which can increase gene transcription through binding transcription factors with RNA polymerase II or via their acetyltransferase activity. CITED4 behaves as transcriptional coactivator for TFAP2/AP-2 and enhances estrogen receptors mediated transactivation. It can disrupt HIF1A -CREBBP interaction for acting as inhibitor of HIF1A mediated transactivation. CITED4 implicates in gene expression regulation during development/differentiation of blood cells, endothelial cells as well as mammary epithelial cells. CITED4 primarily localize to nucleus, however, its cytoplasmatic translocation or loss of nuclear expression has been observed in breast cancer where it is considered a prognostic marker for carcinogenesis. Moreover, in breast cancer its expression is down-regulated and often correlate with elevated HIF1A levsls, tumor growth and angiogenesis.

Alternate Names

cbp/p300-interacting transactivator 4, Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain4,MRG-2, MRG2, MSG1-related protein 2, transcriptional co-activator 4

Gene Symbol

CITED4

Additional CITED4 Products

Product Documents for CITED4 Antibody (HT13-2D6.3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CITED4 Antibody (HT13-2D6.3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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