CLASP2 Antibody (KT68)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23220
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # KT68
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CLASP2 Antibody (KT68)
Immunogen
Clone KT68 was generated from a GST fusion protein containing the N-terminus of mouse CLASP2. This sequence is highly conserved in human and rat CLASP2, and has low homology to CLASP1. [UniProt# Q8BRT1]
Specificity
The antibody detects a 160 kDa doublet corresponding to the molecular mass of CLASP2 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP2 by immunocytochemistry in HeLa and A431 cells.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Scientific Data Images for CLASP2 Antibody (KT68)
Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] -
Western Blot: CLASP2 Antibody (KT68) [NBP3-23220] - Western blot of CLASP2 in mouse brain (lanes 1 & 3) and rat PC12 cells (lanes 2 & 4). The blots were probed with rat monoclonals NBP3-23220 anti-CLASP2 (clone KT68) (lanes 1 & 2) and anti-CLASP2 (clone KT69) (lanes 3 & 4). The antibodies were used at a dilution of 1:500.
Immunocytochemistry/Immunofluorescence: CLASP2 Antibody (KT68) [NBP3-23220] -
Immunocytochemistry/Immunofluorescence: CLASP2 Antibody (KT68) [NBP3-23220] - Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-CLASP2 (NBP3-23220) (middle). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.Applications for CLASP2 Antibody (KT68)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLASP2
Alternate Names
CLIP-associating protein 2, cytoplasmic linker associated protein 2, Cytoplasmic linker-associated protein 2, hOrbit2, KIAA0627, multiple asters (Mast)-like homolog 2, Protein Orbit homolog 2
Gene Symbol
CLASP2
Additional CLASP2 Products
Product Documents for CLASP2 Antibody (KT68)
Product Specific Notices for CLASP2 Antibody (KT68)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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