Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CLASP2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human cytoplasmic linker associated protein 2 using the numbering given in entry BAG11221.1 (GeneID 23122).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22992739).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLASP2 Antibody

Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395] - Detection of Human CLASP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: CLASP2 Antibody [NBP1-21395]

Immunoprecipitation: CLASP2 Antibody [NBP1-21395]

Immunoprecipitation: CLASP2 Antibody [NBP1-21395] - Detection of human CLASP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 (lot NBP1-21395-2) used for IP at 6 ug per reaction. CLASP2 was also immunoprecipitated by a previous lot of this antibody (NBP1-21395-1) and rabbit anti-CLASP2 antibody NBP1-21394. For blotting immunoprecipitated CLASP2, NBP1-21395 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CLASP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 22992739)

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CLASP2

CLASP2 (cytoplasmic linker-associated protein 2) localizes to the kinetochore where it regulates spindle microtubule dynamics and is required for proper chromosome alignment and segregation. CLASP2 is also known as CLIP-associated protein 2, hOrbit2, and KIAA0627.

Alternate Names

CLIP-associating protein 2, cytoplasmic linker associated protein 2, Cytoplasmic linker-associated protein 2, hOrbit2, KIAA0627, multiple asters (Mast)-like homolog 2, Protein Orbit homolog 2

Entrez Gene IDs

23122 (Human)

Gene Symbol

CLASP2

UniProt

Q31073 (Porcine)

Additional CLASP2 Products

Product Documents for CLASP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CLASP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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