Skip to main content

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human cytoplasmic linker associated protein 2 using the numbering given in entry BAG11221.1 (GeneID 23122).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22992739).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLASP2 Antibody - BSA Free

Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395] - Detection of Human CLASP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395]

Western Blot: CLASP2 Antibody [NBP1-21395] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: CLASP2 Antibody [NBP1-21395]

Immunoprecipitation: CLASP2 Antibody [NBP1-21395]

Immunoprecipitation: CLASP2 Antibody [NBP1-21395] - Detection of human CLASP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CLASP2 antibody NBP1-21395 (lot NBP1-21395-2) used for IP at 6 ug per reaction. CLASP2 was also immunoprecipitated by a previous lot of this antibody (NBP1-21395-1) and rabbit anti-CLASP2 antibody NBP1-21394. For blotting immunoprecipitated CLASP2, NBP1-21395 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CLASP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 22992739)

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CLASP2

CLASP2 (cytoplasmic linker-associated protein 2) localizes to the kinetochore where it regulates spindle microtubule dynamics and is required for proper chromosome alignment and segregation. CLASP2 is also known as CLIP-associated protein 2, hOrbit2, and KIAA0627.

Alternate Names

CLIP-associating protein 2, cytoplasmic linker associated protein 2, Cytoplasmic linker-associated protein 2, hOrbit2, KIAA0627, multiple asters (Mast)-like homolog 2, Protein Orbit homolog 2

Entrez Gene IDs

23122 (Human)

Gene Symbol

CLASP2

Additional CLASP2 Products

Product Documents for CLASP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CLASP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...