Claspin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-246
![Western Blot: Claspin Antibody [NB100-246]](https://resources.bio-techne.com/images/products/Claspin-Antibody-Western-Blot-NB100-246-img0004.jpg "Western Blot: Claspin Antibody [NB100-246]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation (Negative), Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Claspin Antibody - BSA Free
Western Blot: Claspin Antibody [NB100-246]
Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: Claspin Antibody [NB100-246]](https://resources.bio-techne.com/images/products/Claspin-Antibody-Western-Blot-NB100-246-img0002.jpg "Western Blot: Claspin Antibody [NB100-246]")
Western Blot: Claspin Antibody [NB100-246]
Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.Applications for Claspin Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:5000
Application Notes
By Western blot, this antibody recognizes bands around 175 kDa representing Claspin. Not suitable for Immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application. **Use NB 100-247 and/or NB 100-248 for Immunoprecipitation.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Claspin
Additional Claspin Products
Product Documents for Claspin Antibody - BSA Free
Product Specific Notices for Claspin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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