Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation (Negative), Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Claspin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Claspin Antibody

Western Blot: Claspin Antibody [NB100-246]

Western Blot: Claspin Antibody [NB100-246]

Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Claspin Antibody [NB100-246]

Western Blot: Claspin Antibody [NB100-246]

Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.

Applications for Claspin Antibody

Application
Recommended Usage

Western Blot

1:1000-1:5000
Application Notes
By Western blot, this antibody recognizes bands around 175 kDa representing Claspin. Not suitable for Immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application. **Use NB 100-247 and/or NB 100-248 for Immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Claspin

Claspin is a cell cycle regulated protein. It plays a critical role in replication checkpoint control and interacts with the checkpoint proteins ATR and Rad9. It also associates with Chk1, but only following replication stress or other types of DNA damage.

Alternate Names

CLSPN

Entrez Gene IDs

63967 (Human)

Gene Symbol

CLSPN

UniProt

AAG24515.1 (Human)

Additional Claspin Products

Product Documents for Claspin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Claspin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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