Claspin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-247
![Western Blot: Claspin Antibody [NB100-247]](https://resources.bio-techne.com/images/products/Claspin-Antibody-Western-Blot-NB100-247-img0028.jpg "Western Blot: Claspin Antibody [NB100-247]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Chimpanzee (100%), Orangutan (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).
Reactivity Notes
Gorilla (100%).
Localization
Nucleus.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Claspin Antibody - BSA Free
Western Blot: Claspin Antibody [NB100-247]
Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: Claspin Antibody [NB100-247]](https://resources.bio-techne.com/images/products/Claspin-Antibody-Immunohistochemistry-NB100-247-img0027.jpg "Immunohistochemistry-Paraffin: Claspin Antibody [NB100-247]")
Immunohistochemistry-Paraffin: Claspin Antibody [NB100-247]
Immunohistochemistry-Paraffin: Claspin Antibody [NB100-247] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: Claspin Antibody [NB100-247]](https://resources.bio-techne.com/images/products/Claspin-Antibody-Immunoprecipitation-NB100-247-img0023.jpg "Immunoprecipitation: Claspin Antibody [NB100-247]")
Immunoprecipitation: Claspin Antibody [NB100-247]
Immunoprecipitation: Claspin Antibody [NB100-247] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Claspin,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for Claspin Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
1 - 5 ug/mg lysate
Western Blot
1:5000-1:20000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Claspin
Additional Claspin Products
Product Documents for Claspin Antibody - BSA Free
Product Specific Notices for Claspin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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