Claspin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-248
![Western Blot: Claspin Antibody [NB100-248]](https://resources.bio-techne.com/images/products/Claspin-Antibody-Western-Blot-NB100-248-img0001.jpg "Western Blot: Claspin Antibody [NB100-248]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 900 and 950 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:31825846).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Claspin Antibody
Western Blot: Claspin Antibody [NB100-248]
Western Blot: Claspin Antibody [NB100-248] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-Claspin antibody NB100-248 (lot number 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Claspin was also immunoprecipitated by a previous lot (lot number 1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).Applications for Claspin Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg of lysate
Western Blot
1:1000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:31825846).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Claspin
Additional Claspin Products
Product Documents for Claspin Antibody
Product Specific Notices for Claspin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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