Claudin-1 Antibody (JE30-33)

Western Blot: Claudin-1 Antibody (JE30-33) [NBP3-32190]

Western Blot: Claudin-1 Antibody (JE30-33) [NBP3-32190] - Western blot analysis of Claudin-1 on different lysates with Rabbit anti-Claudin-1 antibody (NBP3-32190) at 1/2,000 dilution.

Lane 1: A431 cell lysate (20 ug/Lane)
Lane 2: HUVEC cell lysate (20 ug/Lane)
Lane 3: Caco-2 cell lysate (20 ug/Lane)
Lane 4: HEK-293 cell lysate (negative) (20 ug/Lane)
Lane 5: Mouse skin tissue lysate (40 ug/Lane)
Lane 6: Mouse liver tissue lysate (40 ug/Lane)
Lane 7: Rat skin tissue lysate (40 ug/Lane)
Lane 8: Rat liver tissue lysate (40 ug/Lane)

Predicted band size: 23 kDa
Observed band size: 19 kDa

Exposure time: Lane 1-2: 8 seconds; Lane 3-8: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32190) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Claudin-1 Antibody (JE30-33) [NBP3-32190]

Immunohistochemistry: Claudin-1 Antibody (JE30-33) [NBP3-32190] - Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue with Rabbit anti-Claudin-1 antibody (NBP3-32190) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32190) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.