Claudin-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77036

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Mouse, Rat, Canine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Claudin-1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human Claudin-1. The immunogen is located within the last 50 amino acids of Claudin-1. Amino Acid Sequence: PRKTTSYPTPRPYPKPAPS

Reactivity Notes

Porcine reactivity per customer review. Mouse reactivity reported in (PMID: 33392105), Canine reactivity reported in (PMID: 32419902).

Specificity

Human Claudin-1 has one isoform (211aa, 23kD). Mouse Claudin-1 has one isoform (211aa, 23kD) and Rat Claudin-1 also has one isoform (211aa, 23kD). NBP1-77036 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Claudin-1 Antibody - BSA Free

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. The relative expression of claudin-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following publication (https://jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036]

Immunocytochemistry/ Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036]

Immunocytochemistry/Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation of CLDN1 in HepG2 Cells. Analysisof 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 AntibodyBSA Free [NBP1-77036]

Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation in Human Tissue Lysates Loading: 15 ug of lysates per lane.Antibodies: CLDN1, (1 ug/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for Claudin-1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/mL

Western Blot

1-2 ug/ml
Application Notes
Use in ICC/IF reported in multiple pieces of scientific literature. Use in IHC reported in scientific literature (PMID:32067099).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using NBP1-77036 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-1

Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.

Alternate Names

Claudin1, CLD1, CLDN1, SEMP1

Entrez Gene IDs

9076 (Human); 12737 (Mouse); 65129 (Rat)

Gene Symbol

CLDN1

UniProt

O95832

Additional Claudin-1 Products

Product Documents for Claudin-1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Claudin-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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