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Claudin-11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82470

Novus Biologicals, part of Bio-Techne
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NBP1-82470
NBP1-82470-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DAQAFGENRFYYTAGSSSPTHAKSAHV

Reactivity Notes

Rat (89%). Mouse reactivity reported in scientific literature (PMID: 25630681).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Claudin-11 Antibody

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470] - Analysis in human testis and pancreas tissues using NBP1-82470 antibody. Corresponding CLDN11 RNA-seq data are presented for the same tissues.
Western Blot: Claudin-11 Antibody [NBP1-82470]

Western Blot: Claudin-11 Antibody [NBP1-82470]

Western Blot: Claudin-11 Antibody [NBP1-82470] - HUVEC (Human Umbilical Vein Endothelial Cells) lysate. Three dilutions were tested using the WES 12-230 kDa kit (left panel): Lanes 2-3, 1:100; Lanes 4-5, 1:50; and Lanes 6-7, 1:25. The 1:25 dilution was tested again using the 2-40 kDa (right panel). All test lanes utilized 0.2 mg/mL protein concentrations. The WES anti-rabbit antibody kit was used. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.

Applications for Claudin-11 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

Reported in scientific literature (PMID: 26339636)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-82470 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-11

Oligodendrocyte specific protein belongs is a major component of central nervous system myelin that is necessary for normal CNS function. The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, and are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that the Claudin 11 protein determines the permeability between layers of myelin sheaths via focal adhesion and with its expression highly regulated during development, may play an important role in cellular proliferation and migration. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.

Alternate Names

Claudin11, CLDN11, OSP, OTM

Gene Symbol

CLDN11

Additional Claudin-11 Products

Product Documents for Claudin-11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Claudin-11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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