Claudin-3 Antibody (CL13128)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18577

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13128

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Claudin-3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a sythetic peptide for O15551. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Claudin-3 Antibody (CL13128)

Western Blot: Claudin-3 Antibody (CL13128) [NBP3-18577]

Western Blot: Claudin-3 Antibody (CL13128) [NBP3-18577]

Western Blot: Claudin-3 Antibody (CL13128) [NBP3-18577] - Analysis in human cell line MCF-7 and human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: Claudin-3 Antibody (CL13128) [NBP3-18577]

Immunocytochemistry/ Immunofluorescence: Claudin-3 Antibody (CL13128) [NBP3-18577]

Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (CL13128) [NBP3-18577] - Staining of MCF-7 cells using the Anti-CLDN3 monoclonal antibody, showing specific staining in cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Claudin-3 Antibody (CL13128) [NBP3-18577]

Immunohistochemistry-Paraffin: Claudin-3 Antibody (CL13128) [NBP3-18577]

Immunohistochemistry-Paraffin: Claudin-3 Antibody (CL13128) [NBP3-18577] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
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Applications for Claudin-3 Antibody (CL13128)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-3

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin3, CLDN3, CPE-R 2, CPETR2, HRVP1

Gene Symbol

CLDN3

Additional Claudin-3 Products

Product Documents for Claudin-3 Antibody (CL13128)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Claudin-3 Antibody (CL13128)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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