Claudin-3 Antibody (HL1527) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25412
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Rhesus Macaque (90%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1527
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Claudin-3 Antibody (HL1527) - Azide and BSA Free
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Claudin-3. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Claudin-3 Antibody (HL1527) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] -
Immunocytochemistry/Immunofluorescence: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 and HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:500. Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] -
Immunohistochemistry-Paraffin: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon. Claudin 3 stained by Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] -
Western Blot: Claudin-3 Antibody (HL1527) - Azide and BSA Free [NBP3-25412] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1527] (NBP3-25412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for Claudin-3 Antibody (HL1527) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Claudin-3
Alternate Names
Claudin3, CLDN3, CPE-R 2, CPETR2, HRVP1
Gene Symbol
CLDN3
Additional Claudin-3 Products
Product Documents for Claudin-3 Antibody (HL1527) - Azide and BSA Free
Product Specific Notices for Claudin-3 Antibody (HL1527) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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