Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Fish - Danio rerio (Zebrafish)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1E7

Concentration

1 mg/ml

View all Claudin-3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CLDN3 (NP_001297) produced in HEK293T cell.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID:32866532). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Claudin-3 Antibody (OTI1E7)

Western Blot: Claudin-3 Antibody (OTI1E7) [NBP2-46299]

Western Blot: Claudin-3 Antibody (OTI1E7) [NBP2-46299]

Western Blot: Claudin-3 Antibody (1E7) [NBP2-46299] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Claudin-3.
Immunohistochemistry: Claudin-3 Antibody (OTI1E7) [NBP2-46299]

Immunohistochemistry: Claudin-3 Antibody (OTI1E7) [NBP2-46299]

Immunohistochemistry: Claudin-3 Antibody (1E7) [NBP2-46299] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Claudin-3 Antibody (OTI1E7)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:32866532)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Claudin-3

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23.

Alternate Names

Claudin3, CLDN3, CPE-R 2, CPETR2, HRVP1

Gene Symbol

CLDN3

Additional Claudin-3 Products

Product Documents for Claudin-3 Antibody (OTI1E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Claudin-3 Antibody (OTI1E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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