CLIP170 Antibody (CL4851)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61144
![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.bio-techne.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0001.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (97%), Rat (95%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL4851
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for CLIP170 Antibody (CL4851)
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:KNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDET
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CLIP170 Antibody (CL4851)
![Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.bio-techne.com/images/products/CLIP170-Antibody-CL4851-Western-Blot-NBP2-61144-img0010.jpg "Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144]")
Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144]
Western Blot: CLIP170 Antibody (CL4851) [NBP2-61144] - Analysis in human cell line HDLM-2 and human cell line U-2197.![Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.bio-techne.com/images/products/CLIP170-Antibody-CL4851-Immunocytochemistry-Immunofluorescence-NBP2-61144-img0011.jpg "Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144]")
Immunocytochemistry/ Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144]
Immunocytochemistry/Immunofluorescence: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of A549 cells using the Anti-CLIP1 monoclonal antibody, showing specific staining in microtubule ends in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.bio-techne.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0004.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]
Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144] - Staining of human kidney shows moderate to strong cytoplasmic immunoreactivity in renal tubules.![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.bio-techne.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0002.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
![Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]](https://resources.bio-techne.com/images/products/CLIP170-Antibody-CL4851-Immunohistochemistry-Paraffin-NBP2-61144-img0003.jpg "Immunohistochemistry-Paraffin: CLIP170 Antibody (CL4851) [NBP2-61144]")
Applications for CLIP170 Antibody (CL4851)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:10000 - 1:20000
Immunohistochemistry-Paraffin
1:10000 - 1:20000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLIP170
Alternate Names
CAP-GLY domain containing linker protein 1, CLIP, CLIP170, CLIP-170Reed-Sternberg intermediate filament-associated protein, CYLN1MGC131604, cytoplasmic linker 1, Cytoplasmic linker protein 1, Cytoplasmic linker protein 170 alpha-2, Reed-Steinberg cell-expressed intermediate filament-associated protein, Restin, restin (Reed-Steinberg cell-expressed intermediate filament-associated protein), RSNCAP-Gly domain-containing linker protein 1
Gene Symbol
CLIP1
Additional CLIP170 Products
Product Documents for CLIP170 Antibody (CL4851)
Product Specific Notices for CLIP170 Antibody (CL4851)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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