CNOT2 Antibody (OTI5A12) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71202
![Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]](https://resources.bio-techne.com/images/products/CNOT2-Antibody-OTI5A12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71202-img0003.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]")
![Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]](https://resources.bio-techne.com/images/products/CNOT2-Antibody-OTI5A12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71202-img0005.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]")
![Western Blot: CNOT2 Antibody (OTI5A12)Azide and BSA Free [NBP2-71202]](https://resources.bio-techne.com/images/products/CNOT2-Antibody-OTI5A12-Azide-and-BSA-Free-Western-Blot-NBP2-71202-img0001.jpg "Western Blot: CNOT2 Antibody (OTI5A12)Azide and BSA Free [NBP2-71202]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5A12
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human CNOT2(NP_055330) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CNOT2 Antibody (OTI5A12) - Azide and BSA Free
Western Blot: CNOT2 Antibody (OTI5A12)Azide and BSA Free [NBP2-71202]
Western Blot: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT2.![Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]](https://resources.bio-techne.com/images/products/CNOT2-Antibody-OTI5A12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71202-img0004.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]")
Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]
Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)![Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]](https://resources.bio-techne.com/images/products/CNOT2-Antibody-OTI5A12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71202-img0002.jpg "Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]")
Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202]
Immunohistochemistry: CNOT2 Antibody (OTI5A12) - Azide and BSA Free [NBP2-71202] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)Applications for CNOT2 Antibody (OTI5A12) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CNOT2
Alternate Names
CCR4-associated factor 2, CCR4-NOT transcription complex, subunit 2, CDC36FLJ26456, negative regulator of transcription 2, NOT2CCR4-NOT transcription complex subunit 2, NOT2H
Gene Symbol
CNOT2
Additional CNOT2 Products
Product Documents for CNOT2 Antibody (OTI5A12) - Azide and BSA Free
Product Specific Notices for CNOT2 Antibody (OTI5A12) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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