CNPase Antibody (1H10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50031
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1H10
Concentration
1 mg/ml
Product Summary for CNPase Antibody (1H10)
Immunogen
Full length human recombinant CNPase protein expressed in and purified from E. coli. [UniProt# P09543]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46,48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CNPase Antibody (1H10)
Western Blot: CNPase Antibody (1H10) [NBP2-50031]
Western Blot: CNPase Antibody (1H10) [NBP2-50031] - Western blot analysis of different tissue lysates using mouse mAb to CNP, NBP2-50031, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. A doublet of bands at 46 and 48kDa correspond to the major isotypes of the CNP protein.Immunocytochemistry/ Immunofluorescence: CNPase Antibody (1H10) [NBP2-50031]
Immunocytochemistry/Immunofluorescence: CNPase Antibody (1H10) [NBP2-50031] - Mixed neuron-glial cell cultures stained with NBP2-50031 (red) and polyclonal antibody against GFAP (green, NB300-141). NBP2-50031 stains strongly in oligodendrocytes, whereas GFAP labels only the intermediate filaments in astrocytes. Blue is DNA staining.Immunohistochemistry Free-Floating: CNPase Antibody (1H10) [NBP2-50031]
Immunohistochemistry Free-Floating: CNPase Antibody (1H10) [NBP2-50031] - Analysis of rat cerebellum section stained with mouse mAb to CNP, NBP2-50031, dilution 1:500 in green and costained with chicken pAb to NF-M, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNP antibody specifically stains oligodendrocytes, cells that create the myelin sheath around axons. NF-M antibody labels axons of neuronal cells.Applications for CNPase Antibody (1H10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Western Blot
1:5000 - 1:20000
Application Notes
In western blot, double bands may be detected at 46 and 48 kDa corresponding to the major isotypes of the CNP protein.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CNPase
Alternate Names
2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37
Gene Symbol
CNP
UniProt
Additional CNPase Products
Product Documents for CNPase Antibody (1H10)
Product Specific Notices for CNPase Antibody (1H10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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