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CNPase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85995

Novus Biologicals, part of Bio-Techne
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NBP1-85995
NBP1-85995-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQW

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Marker

Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNPase Antibody

Western Blot: CNPase Antibody [NBP1-85995]

Western Blot: CNPase Antibody [NBP1-85995]

Western Blot: CNPase Antibody [NBP1-85995] - Analysis using Anti-CNP antibody NBP1-85995 (A) shows similar pattern to independent antibody NBP1-85999 (B).
Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85995]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85995]

Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85995] - Staining in human cerebral cortex and pancreas tissues using anti-CNP antibody. Corresponding CNP RNA-seq data are presented for the same tissues.
Western Blot: CNPase Antibody [NBP1-85995]

Western Blot: CNPase Antibody [NBP1-85995]

Western Blot: CNPase Antibody [NBP1-85995] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue

Applications for CNPase Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

Additional CNPase Products

Product Documents for CNPase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CNPase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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